TY  - JOUR
A1  - Puntigam, Lisa K.
A1  - Jeske, Sandra S.
A1  - Götz, Marlies
A1  - Greiner, Jochen
A1  - Laban, Simon
A1  - Theodoraki, Marie-Nicole
A1  - Doescher, Johannes
A1  - Weissinger, Stephanie E.
A1  - Brunner, Cornelia
A1  - Hoffmann, Thomas K.
A1  - Schuler, Patrick J.
T1  - Immune checkpoint expression on immune cells of HNSCC patients and modulation by chemo- and immunotherapy
T2  - International Journal of Molecular Sciences
N2  - Endogenous control mechanisms, including immune checkpoints and immunosuppressive cells, are exploited in the process of tumorigenesis to weaken the anti-tumor immune response. Cancer treatment by chemotherapy or immune checkpoint inhibition can lead to changes of checkpoint expression, which influences therapy success. Peripheral blood lymphocytes (PBL) and tumor-infiltrating lymphocytes (TIL) were isolated from head and neck squamous cell carcinoma (HNSCC) patients (n = 23) and compared to healthy donors (n = 23). Immune checkpoint expression (programmed cell death ligand 1 (PD-1), tumor necrosis factor receptor (TNFR)-related (GITR), CD137, tumor necrosis factor receptor superfamily member 4 (TNFRSF4) (OX40), t-cell immunoglobulin and mucin-domain containing-3 (TIM3), B- and T-lymphocyte attenuator (BTLA), lymphocyte-activation gene 3 (LAG3)) was determined on immune cells by flow cytometry. PD-L1 expression was detected on tumor tissue by immunohistochemistry. Immune cells were treated with immuno- and chemotherapeutics to investigate treatment-specific change in immune checkpoint expression, in vitro. Specific changes of immune checkpoint expression were identified on PBL and TIL of HNSCC patients compared to healthy donors. Various chemotherapeutics acted differently on the expression of immune checkpoints. Changes of checkpoint expression were significantly less pronounced on regulatory T cells compared to other lymphocyte populations. Nivolumab treatment significantly reduced the receptor PD-1 on all analyzed T cell populations, in vitro. The specific immune checkpoint expression patterns in HNSCC patients and the investigated effects of immunomodulatory agents may improve the development and efficacy of targeted immunotherapy.
KW  - Inorganic Chemistry
KW  - Organic Chemistry
KW  - Physical and Theoretical Chemistry
KW  - Computer Science Applications
KW  - Spectroscopy
KW  - Molecular Biology
KW  - General Medicine
KW  - Catalysis
Y1  - 2020
UR  - https://opus.bibliothek.uni-augsburg.de/opus4/frontdoor/index/index/docId/101392
UR  - https://nbn-resolving.org/urn:nbn:de:bvb:384-opus4-1013927
SN  - 1422-0067
VL  - 21
IS  - 15
SP  - 5181
PB  - MDPI AG
ER  -