TY  - JOUR
A1  - Loch, Florian N.
A1  - Kamphues, Carsten
A1  - Menzel, Peter
A1  - Schwarzer, Rolf
A1  - Beyer, Katharina
A1  - Schineis, Christian
T1  - Mucosal microbiome of surgically treated terminal ileal Crohn's disease
T2  - Frontiers in Cellular and Infection Microbiology
N2  - Crohn’s disease (CD) is associated with changes in the microbiome. The role of these changes and their precise association with disease course and activity remain ambiguous. In this prospective single-center study, the mucosal microbiome of surgical CD and non-CD patients was compared at the time of surgery. Microbial analyses were individually performed for ileal and colonic tissue samples obtained during surgery using 16S-rRNA-gene amplicon sequencing. Three groups out of the 46 included patients were formed: 1) a study group of CD of patients who received ileocecal resection due to CD involvement (CD study, n=10); 2) a control group of non-CD of patients who received intestinal resection due to indications other than CD (non-CD control, n=27); and 3) a second control group of CD who underwent resection of the intestine not affected by CD (CD non-affected control, n=9). Species richness and Shannon diversity were not different between all formed groups and regions analyzed (p>0.05). Several significant taxonomic differences were seen at the phylum-, order-, and genus-levels between the formed groups, such as a decrease of Firmicutes (phylum-level) and an increase of Bacteroides and Escherichia/Shigella/Pseudescherichia (genus-level) in CD study – colon vs. the non-CD control – colon (p ≤ 0.05). The CD non-affected control presented the largest amount of differentially abundant taxa in comparison to the other groups. These results underline that CD is accompanied by changes in affected and non-affected intestinal regions compared to non-CD controls. This study contributes the mucosal microbiome of a well-defined subset of surgical CD patients without confounding aspects of the fecal microbiome or regional microbial differences to the existing literature.
Y1  - 2024
UR  - https://opus.bibliothek.uni-augsburg.de/opus4/frontdoor/index/index/docId/123724
UR  - https://nbn-resolving.org/urn:nbn:de:bvb:384-opus4-1237245
SN  - 2235-2988
VL  - 13
SP  - 1324668
PB  - Frontiers Media SA
ER  -