TY  - JOUR
A1  - Loch, Florian N.
A1  - Klein, Oliver
A1  - Beyer, Katharina
A1  - Klauschen, Frederick
A1  - Schineis, Christian
A1  - Lauscher, Johannes C.
A1  - Margonis, Georgios A.
A1  - Degro, Claudius E.
A1  - Rayya, Wael
A1  - Kamphues, Carsten
T1  - Peptide signatures for prognostic markers of pancreatic cancer by MALDI mass spectrometry imaging
T2  - Biology
N2  - Despite the overall poor prognosis of pancreatic cancer there is heterogeneity in clinical courses of tumors not assessed by conventional risk stratification. This yields the need of additional markers for proper assessment of prognosis and multimodal clinical management. We provide a proof of concept study evaluating the feasibility of Matrix-assisted laser desorption/ionization (MALDI) mass spectrometry imaging (MSI) to identify specific peptide signatures linked to prognostic parameters of pancreatic cancer. On 18 patients with exocrine pancreatic cancer after tumor resection, MALDI imaging analysis was performed additional to histopathological assessment. Principal component analysis (PCA) was used to explore discrimination of peptide signatures of prognostic histopathological features and receiver operator characteristic (ROC) to identify which specific m/z values are the most discriminative between the prognostic subgroups of patients. Out of 557 aligned m/z values discriminate peptide signatures for the prognostic histopathological features lymphatic vessel invasion (pL, 16 m/z values, eight proteins), nodal metastasis (pN, two m/z values, one protein) and angioinvasion (pV, 4 m/z values, two proteins) were identified. These results yield proof of concept that MALDI-MSI of pancreatic cancer tissue is feasible to identify peptide signatures of prognostic relevance and can augment risk assessment.
Y1  - 2021
UR  - https://opus.bibliothek.uni-augsburg.de/opus4/frontdoor/index/index/docId/123874
UR  - https://nbn-resolving.org/urn:nbn:de:bvb:384-opus4-1238746
SN  - 2079-7737
VL  - 10
IS  - 10
SP  - 1033
PB  - MDPI AG
CY  - Basel
ER  -