TY  - JOUR
A1  - Einhauser, Sebastian
A1  - Asam, Claudia
A1  - Weps, Manuela
A1  - Senninger, Antonia
A1  - Peterhoff, David
A1  - Bauernfeind, Stilla
A1  - Asbach, Benedikt
A1  - Carnell, George William
A1  - Heeney, Jonathan Luke
A1  - Wytopil, Monika
A1  - Fuchs, André
A1  - Messmann, Helmut
A1  - Prelog, Martina
A1  - Liese, Johannes
A1  - Jeske, Samuel D.
A1  - Protzer, Ulrike
A1  - Hoelscher, Michael
A1  - Geldmacher, Christof
A1  - Überla, Klaus
A1  - Steiniger, Philipp
A1  - Wagner, Ralf
A1  - Ebigbo, Alanna
A1  - Römmele, Christoph
A1  - Ullrich, Maximilian
A1  - Freitag, Marie
A1  - Traidl-Hoffmann, Claudia
A1  - Goekkaya, Mehmet
A1  - Metz, Aline
A1  - Holetschek, Corinna
A1  - Neumann, Avidan
A1  - Neumann, Reinhard
A1  - Kling, Elisabeth
A1  - Pruteanu, Mihail
A1  - Wibmer, Thomas
A1  - Rost, Susanne
A1  - Beileke, Stephanie
A1  - Müller-Schmucker, Sandra
A1  - Korn, Klaus
A1  - Hastreiter, Tamara
A1  - Fraedrich, Kirsten
A1  - Obergfäll, Debora
A1  - Neumann, Frank
A1  - Kuhn, Claudia
A1  - Günther, Katja
A1  - Friedrich, Elke
A1  - Wieser, Andreas
A1  - Janke, Christian
A1  - Plank, Michael
A1  - Guggenbühl, Jessica
A1  - Reinkemeyer, Christina
A1  - Noreña, Ivan
A1  - Castelletti, Noemi
A1  - Acero, Raquel Rubio
A1  - Ahmed, M. I. M.
A1  - Diepers, Paulina
A1  - Eser, Tabea M.
A1  - Fuchs, Anna
A1  - Baranov, Olga
A1  - Bauer, Bernadette
A1  - Wang, Danni
A1  - Paunovic, Ivana
A1  - Christa, Catharina
A1  - Tinnefeld, Kathrin
A1  - Vu, Martin
A1  - Willmann, Annika
A1  - Roggendorf, Hedwig
A1  - Körber, Nina
A1  - Bauer, Tanja
A1  - Gleich, Sabine
A1  - Ebner, Antonia
A1  - de Schultz, Maria José
A1  - Rajes, Cedric
A1  - Al Wafai, Aya
A1  - Brenner, David
A1  - Sicheneder, Laura
A1  - Berr, Melanie
A1  - Schütz, Anja
A1  - Hiergeist, Andreas
A1  - Gessner, André
A1  - Schmidt, Barbara
A1  - Niller, Hans-Helmut
A1  - Wenzel, Jürgen
A1  - Biermeier, Daniela
A1  - Lampl, Benedikt
A1  - Rothe, Ulrich
A1  - Gleißner, Ute
A1  - Brückner, Susanne
A1  - Treml, Michaela
A1  - Schedl, Holger
A1  - Biermaier, Beate
A1  - Achatz, Markus
A1  - Hierhammer, Daniela
A1  - Englhardt, Johanna
A1  - Scheidl, Werner
A1  - Jeyaraman, Sivaji
A1  - Schutt, Barbara
A1  - Almanzar, Giovanni
A1  - Schwägerl, Valeria
A1  - Bley, Julia
A1  - Vogt, Tim
A1  - Kousha, Kimia
A1  - Ziegler, Lars
A1  - Stein, Astrid
A1  - Förg, Franziska
A1  - Löw, Johann
A1  - Finkenberg, Barbara
A1  - Pollak, Dennis
A1  - Zamzow, Alexander
A1  - Eberbach, Nicole
A1  - Balkie, Lara
A1  - Kretzschmann, Tanja
A1  - Gehrig, Matthias
A1  - Bandorf, Matthias
A1  - Keck, Kilian
A1  - Allmanritter, Jan
A1  - Rafique, Shahid
A1  - Finster, Mona
A1  - Baumgart, Ingo
A1  - Heumüller-Klug, Sabine
A1  - Koglin, Hans-Jürgen
A1  - Gefeller, Olaf
A1  - Gall, Christine
A1  - Pfahlberg, Annette B.
A1  - Kaiser, Isabelle
A1  - Scheidt, Jörg
A1  - Drescher, Johannes
A1  - Siebenhaar, Yannic
A1  - Wogenstein, Florian
A1  - Reinel, Dirk
A1  - Weber, Beatrix
A1  - Zarzitzky, Fabian
A1  - Liebl, Bernhard
A1  - Herr, Caroline
A1  - Katz, Katharina
A1  - Sing, Andreas
A1  - Dangel, Alexandra
T1  - Longitudinal effects of SARS-CoV-2 breakthrough infection on imprinting of neutralizing antibody responses
T2  - eBioMedicine
N2  - Background
The impact of the infecting SARS-CoV-2 variant of concern (VOC) and the vaccination status was determined on the magnitude, breadth, and durability of the neutralizing antibody (nAb) profile in a longitudinal multicentre cohort study.

Methods
173 vaccinated and 56 non-vaccinated individuals were enrolled after SARS-CoV-2 Alpha, Delta, or Omicron infection and visited four times within 6 months and nAbs were measured for D614G, Alpha, Delta, BA.1, BA.2, BA.5, BQ.1.1, XBB.1.5 and JN.1.

Findings
Magnitude-breadth-analysis showed enhanced neutralization capacity in vaccinated individuals against multiple VOCs. Longitudinal analysis revealed sustained neutralization magnitude-breadth after antigenically distant Delta or Omicron breakthrough infection (BTI), with triple-vaccinated individuals showing significantly elevated titres and improved breadth. Antigenic mapping and antibody landscaping revealed initial boosting of vaccine-induced WT-specific responses after BTI, a shift in neutralization towards infecting VOCs at peak responses and an immune imprinted bias towards dominating WT immunity in the long-term. Despite that bias, machine-learning models confirmed a sustained shift of the immune-profiles following BTI.

Interpretation
In summary, our longitudinal analysis revealed delayed and short lived nAb shifts towards the infecting VOC, but an immune imprinted bias towards long-term vaccine induced immunity after BTI.

Funding
This work was funded by the Bavarian State Ministry of Science and the Arts for the CoVaKo study and the ForCovid project. The funders had no influence on the study design, data analysis or data interpretation.
Y1  - 2024
UR  - https://opus.bibliothek.uni-augsburg.de/opus4/frontdoor/index/index/docId/116770
UR  - https://nbn-resolving.org/urn:nbn:de:bvb:384-opus4-1167701
SN  - 2352-3964
VL  - 110
SP  - 105438
PB  - Elsevier
CY  - Amsterdam
ER  -