Th2/Th17 cell associated cytokines found in seroma fluids after breast cancer surgery

  • Purpose The development of a seroma after breast cancer surgery is a common postoperative complication seen after simple mastectomy and axillary surgery. We could recently demonstrate that breast cancer patients undergoing a simple mastectomy with subsequent seroma formation developed a T-helper cell increase within the aspirated fluid measured by flow cytometry. The same study revealed a Th2 and/or a Th17 immune response in peripheral blood and seroma fluid of the same patient. Based on these results and within the same study population, we now analyzed the Th2/Th17 cell associated cytokine content as well as the best known clinical important cytokine IL-6. Methods Multiplex cytokine measurements (IL-4, IL-5, IL-13, IL-10, IL-17, and IL-22) were done on 34 seroma fluids (Sf) after fine needle aspiration of patients who developed a seroma after a simple mastectomy. Serum of the same patient (Sp) and that of healthy volunteers (Sc) were used as controls. Results We found the SfPurpose The development of a seroma after breast cancer surgery is a common postoperative complication seen after simple mastectomy and axillary surgery. We could recently demonstrate that breast cancer patients undergoing a simple mastectomy with subsequent seroma formation developed a T-helper cell increase within the aspirated fluid measured by flow cytometry. The same study revealed a Th2 and/or a Th17 immune response in peripheral blood and seroma fluid of the same patient. Based on these results and within the same study population, we now analyzed the Th2/Th17 cell associated cytokine content as well as the best known clinical important cytokine IL-6. Methods Multiplex cytokine measurements (IL-4, IL-5, IL-13, IL-10, IL-17, and IL-22) were done on 34 seroma fluids (Sf) after fine needle aspiration of patients who developed a seroma after a simple mastectomy. Serum of the same patient (Sp) and that of healthy volunteers (Sc) were used as controls. Results We found the Sf to be highly cytokine rich. Almost all analyzed cytokines were significantly higher in abundance in the Sf compared to Sp and Sc, especially IL-6, which promotes Th17 differentiation as well as suppresses Th1 differentiation in favor of Th2 development. Conclusion Our Sf cytokine measurements reflect a local immune event. In contrast, former study results on T-helper cell populations in both Sf and Sp tend to demonstrate a systemic immune process.show moreshow less

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Metadaten
Author:Nicole Pochert, Mariella Schneider, Melitta B. Köpke, Mathis Wild, Angelika Mattmer, Jacqueline Sagasser, Monika M. GolasORCiDGND, Maggie Banys-Paluchowski, Aline Metz, Ludwig Christian HinskeORCiDGND, Matthias Reiger, Udo JeschkeORCiDGND, Christian DanneckerORCiDGND, Avidan NeumannGND, Claudia Traidl-HoffmannORCiDGND, Michael Untch, Thorsten Kühn, Nina DitschGND
URN:urn:nbn:de:bvb:384-opus4-1049930
Frontdoor URLhttps://opus.bibliothek.uni-augsburg.de/opus4/104993
ISSN:1432-0711OPAC
Parent Title (English):Archives of Gynecology and Obstetrics
Publisher:Springer Science and Business Media LLC
Place of publication:Berlin
Type:Article
Language:English
Year of first Publication:2023
Publishing Institution:Universität Augsburg
Release Date:2023/06/21
Tag:Obstetrics and Gynecology; General Medicine
Volume:308
First Page:1621
Last Page:1627
DOI:https://doi.org/10.1007/s00404-023-07074-w
Institutes:Medizinische Fakultät
Medizinische Fakultät / Universitätsklinikum
Medizinische Fakultät / Lehrstuhl für Frauenheilkunde
Medizinische Fakultät / Professur für Operative und Konservative Senologie
Medizinische Fakultät / Lehrstuhl für Umweltmedizin
Medizinische Fakultät / Lehrstuhl für Strahlentherapie
Medizinische Fakultät / Lehrstuhl für Humangenetik
Medizinische Fakultät / Lehrstuhl für Datenmanagement und Clinical Decision Support
Nachhaltigkeitsziele
Nachhaltigkeitsziele / Ziel 3 - Gesundheit und Wohlergehen
Dewey Decimal Classification:6 Technik, Medizin, angewandte Wissenschaften / 61 Medizin und Gesundheit / 610 Medizin und Gesundheit
Licence (German):CC-BY 4.0: Creative Commons: Namensnennung (mit Print on Demand)