The E3 ubiquitin ligase MID1 catalyzes ubiquitination and cleavage of Fu

  • SHH (Sonic Hedgehog)-GLI signaling plays an important role during embryogenesis and in tumorigenesis. The survival and growth of several types of cancer depend on autonomously activated SHH-GLI signaling. A protein complex containing the ubiquitin ligase MID1 and protein phosphatase 2A regulates the nuclear localization and transcriptional activity of GLI3, a transcriptional effector molecule of SHH, in cancer cell lines with autonomously activated SHH signaling. However, the exact molecular mechanisms that mediate the interaction between MID1 and GLI3 remained unknown. Here, we show that MID1 catalyzes the ubiquitination and proteasomal cleavage of the GLI3 regulator Fu. Our data suggest that Fu ubiquitination and cleavage is one of the key elements connecting the MID1-PP2A protein complex with GLI3 activity control.

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Author:Susann Schweiger, Stephanie Dorn, Melanie Fuchs, Andrea Köhler, Frank MatthesORCiDGND, Eva-Christina Müller, Erich Wanker, Rainer Schneider, Sybille Krauß
Frontdoor URL
Parent Title (English):Journal of Biological Chemistry
Publisher:Elsevier BV
Year of first Publication:2014
Publishing Institution:Universität Augsburg
Release Date:2023/11/24
Tag:Cell Biology; Molecular Biology; Biochemistry
First Page:31805
Last Page:31817
Institutes:Medizinische Fakultät
Medizinische Fakultät / Professur für Physiologie (Meissner)
Dewey Decimal Classification:6 Technik, Medizin, angewandte Wissenschaften / 61 Medizin und Gesundheit / 610 Medizin und Gesundheit
Licence (German):CC-BY 4.0: Creative Commons: Namensnennung (mit Print on Demand)