Optical investigation of individual red blood cells for determining cell count and cellular hemoglobin concentration in a microfluidic channel
- We demonstrate a blood analysis routine by observing red blood cells through light and digital holographic microscopy in a microfluidic channel. With this setup a determination of red blood cell (RBC) concentration, the mean corpuscular volume (MCV), and corpuscular hemoglobin concentration mean (CHCM) is feasible. Cell count variations in between measurements differed by 2.47% with a deviation of −0.26×106 μL to the reference value obtained from the Siemens ADVIA 2120i. Measured MCV values varied by 2.25% and CHCM values by 3.78% compared to the reference ADVIA measurement. Our results suggest that the combination of optical analysis with microfluidics handling provides a promising new approach to red blood cell counts.
| Author: | Ann-Kathrin Reichenwallner, Esma Vurmaz, Kristina Battis, Laura Handl, Helin Üstün, Tivadar Mach, Gabriele Hörnig, Jan LipfertORCiDGND, Lukas Richter |
|---|---|
| URN: | urn:nbn:de:bvb:384-opus4-1142265 |
| Frontdoor URL | https://opus.bibliothek.uni-augsburg.de/opus4/114226 |
| ISSN: | 2072-666XOPAC |
| Parent Title (English): | Micromachines |
| Publisher: | MDPI AG |
| Place of publication: | Basel |
| Type: | Article |
| Language: | English |
| Year of first Publication: | 2021 |
| Publishing Institution: | Universität Augsburg |
| Release Date: | 2024/07/22 |
| Volume: | 12 |
| Issue: | 4 |
| First Page: | 358 |
| DOI: | https://doi.org/10.3390/mi12040358 |
| Institutes: | Mathematisch-Naturwissenschaftlich-Technische Fakultät |
| Mathematisch-Naturwissenschaftlich-Technische Fakultät / Institut für Physik | |
| Mathematisch-Naturwissenschaftlich-Technische Fakultät / Institut für Physik / Lehrstuhl für Experimentalphysik I | |
| Dewey Decimal Classification: | 5 Naturwissenschaften und Mathematik / 53 Physik / 530 Physik |
| Licence (German): |  CC-BY 4.0: Creative Commons: Namensnennung |
