Analysis of small-angle X-ray scattering data of protein–detergent complexes by singular value decomposition
- Small-angle X-ray scattering can be a valuable tool in the structural characterization of membrane protein–detergent complexes (PDCs). However, a major challenge is to separate the PDC scattering signal from that of the `empty' detergent micelle in a protein–detergent mixture. We briefly review an approach that allows approximate determination of the PDC scattering signal at low momentum transfer and present a novel approach that employs a singular value decomposition (SVD) and fitting of scattering data collected at different protein–detergent stoichiometries. The SVD approach allows the scattering profile for the PDC over the entire measured momentum transfer range to be obtained, it is applicable to strongly scattering detergents and can take into account interparticle interference. The two approaches are contrasted and an application to the membrane protein TM0026 from Thermotoga maritima is presented.
| Author: | Jan LipfertORCiDGND, Linda Columbus, Vincent B. Chu, Sebastian Doniach |
|---|---|
| URN: | urn:nbn:de:bvb:384-opus4-1144929 |
| Frontdoor URL | https://opus.bibliothek.uni-augsburg.de/opus4/114492 |
| ISSN: | 0021-8898OPAC |
| Parent Title (English): | Journal of Applied Crystallography |
| Publisher: | International Union of Crystallography (IUCr) |
| Type: | Article |
| Language: | English |
| Year of first Publication: | 2007 |
| Publishing Institution: | Universität Augsburg |
| Release Date: | 2024/07/31 |
| Volume: | 40 |
| Issue: | s1 |
| First Page: | s235 |
| Last Page: | s239 |
| DOI: | https://doi.org/10.1107/s0021889807005791 |
| Institutes: | Mathematisch-Naturwissenschaftlich-Technische Fakultät |
| Mathematisch-Naturwissenschaftlich-Technische Fakultät / Institut für Physik | |
| Mathematisch-Naturwissenschaftlich-Technische Fakultät / Institut für Physik / Lehrstuhl für Experimentalphysik I | |
| Dewey Decimal Classification: | 5 Naturwissenschaften und Mathematik / 53 Physik / 530 Physik |
| Licence (German): |  CC-BY 4.0: Creative Commons: Namensnennung |
