Rapid quantification of free and esterified phytosterols in human serum using APPI-LC-MS/MS
- A novel analytical platform based on liquid chromatography and tandem mass spectrometry using atmospheric pressure photoionization was applied for the simultaneous quantification of free and esterified β-sitosterol, campesterol, brassicasterol, and stigmasterol. The total time for sample pretreatment and analysis could be reduced from ∼3 h [gas chromatography-mass spectrometry (GC-MS)] to 15 min. The detection limits of the different phytosterols ranged between 0.25 and 0.68 μg/l. Linear ranges were between 1 and 1,000 μg/l. The within-run and between-run variabilities ranged between 1.4% and 9.9%. The analytical sensitivity was at least 150-fold higher compared with GC-MS. Our new method allows a rapid and simultaneous determination of free and esterified phytosterols in serum.
| Author: | Jan Lembcke, Uta Ceglarek, Georg Martin Fiedler, Sven Baumann, Alexander LeichtleORCiDGND, Joachim Thiery |
|---|---|
| URN: | urn:nbn:de:bvb:384-opus4-1245032 |
| Frontdoor URL | https://opus.bibliothek.uni-augsburg.de/opus4/124503 |
| ISSN: | 0022-2275OPAC |
| Parent Title (English): | Journal of Lipid Research |
| Publisher: | Elsevier BV |
| Place of publication: | Amsterdam |
| Type: | Article |
| Language: | English |
| Year of first Publication: | 2005 |
| Publishing Institution: | Universität Augsburg |
| Release Date: | 2025/08/19 |
| Volume: | 46 |
| Issue: | 1 |
| First Page: | 21 |
| Last Page: | 26 |
| DOI: | https://doi.org/10.1194/jlr.c400004-jlr200 |
| Institutes: | Medizinische Fakultät |
| Medizinische Fakultät / Universitätsklinikum | |
| Medizinische Fakultät / Professur für Laboratoriumsmedizin | |
| Dewey Decimal Classification: | 6 Technik, Medizin, angewandte Wissenschaften / 61 Medizin und Gesundheit / 610 Medizin und Gesundheit |
| Licence (German): |  CC-BY 4.0: Creative Commons: Namensnennung |
