Does centrifugation matter? Centrifugal force and spinning time alter the plasma metabolome

  • Background Centrifugation is an indispensable procedure for plasma sample preparation, but applied conditions can vary between labs. Aim Determine whether routinely used plasma centrifugation protocols (1500×g 10 min; 3000×g 5 min) influence non-targeted metabolomic analyses. Methods Nuclear magnetic resonance spectroscopy (NMR) and High Resolution Mass Spectrometry (HRMS) data were evaluated with sparse partial least squares discriminant analyses and compared with cell count measurements. Results Besides significant differences in platelet count, we identified substantial alterations in NMR and HRMS data related to the different centrifugation protocols. Conclusion Already minor differences in plasma centrifugation can significantly influence metabolomic patterns and potentially bias metabolomics studies.

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Metadaten
Author:Dorothea Lesche, Roland Geyer, Daniel Lienhard, Christos T. Nakas, Gaëlle Diserens, Peter Vermathen, Alexander B. LeichtleORCiDGND
URN:urn:nbn:de:bvb:384-opus4-1246396
Frontdoor URLhttps://opus.bibliothek.uni-augsburg.de/opus4/124639
ISSN:1573-3882OPAC
ISSN:1573-3890OPAC
Parent Title (English):Metabolomics
Publisher:Springer Science and Business Media LLC
Place of publication:Berlin
Type:Article
Language:English
Year of first Publication:2016
Publishing Institution:Universität Augsburg
Release Date:2025/08/22
Volume:12
Issue:10
First Page:159
DOI:https://doi.org/10.1007/s11306-016-1109-3
Institutes:Medizinische Fakultät
Medizinische Fakultät / Universitätsklinikum
Medizinische Fakultät / Professur für Laboratoriumsmedizin
Dewey Decimal Classification:6 Technik, Medizin, angewandte Wissenschaften / 61 Medizin und Gesundheit / 610 Medizin und Gesundheit
Licence (German):License LogoCC-BY 4.0: Creative Commons: Namensnennung (mit Print on Demand)

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