- Here we provide data on lake sediment metabarcoding on sedimentary ancient DNA (sedaDNA) from five lake cores from Siberia. Our dataset includes 70 sediment core samples spanning the last about 47.000 years. We applied a PCR-based approach called metabarcoding to amplify specific DNA target sequences: for fungus metabarcoding, we used the ITS marker alongside with the p6 loop trnL chloroplast marker for vegetation metabarcoding. The amplicons were analysed towards their composition with Next Generation Sequencing on 5 sequencing runs (APMG-17, APMG-20 and ATBH-2 for the fungus data; APMG-12 and APMG-29 for the vegetation). The data were used in the publication of von Hippel et al. (2022, Quaternary Science Reviews, accepted). All samples marked with asterisks in the *samples_ages files were not included in the publication.