Acetylcholinesterase activity influenced by lipid membrane area and surface acoustic waves

  • According to the current model of nerve propagation, the function of acetylcholinesterase (AChE) is to terminate synaptic transmission of nerve signals by hydrolyzing the neurotransmitter acetylcholine (ACh) in the synaptic cleft to acetic acid (acetate) and choline. However, extra-synaptic roles, which are known as ‘non-classical’ roles, have not been fully elucidated. Here, we measured AChE activity with the enzyme bound to lipid membranes of varying area per enzyme in vitro using the Ellman assay. We found that the activity was not affected by density fluctuations in a supported lipid bilayer (SLB) induced by standing surface acoustic waves. Nevertheless, we found twice as high activity in the presence of small unilamellar vesicles (SUV) compared to lipid-free samples. We also showed that the increase in activity scaled with the available membrane area per enzyme.

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Author:Lukas G. Schnitzler, Kathrin Baumgartner, Anna Kolb, Benedikt Braun, Christoph WesterhausenORCiDGND
Frontdoor URL
Parent Title (English):Micromachines
Date of first Publication:2022/02/11
Publishing Institution:Universität Augsburg
Release Date:2022/03/28
Tag:acetylcholinesterase; enzyme activity; Ellman assay; lipid membrane; surface acoustic waves
First Page:287
Institutes:Mathematisch-Naturwissenschaftlich-Technische Fakultät
Fakultätsübergreifende Institute und Einrichtungen
Mathematisch-Naturwissenschaftlich-Technische Fakultät / Institut für Physik
Medizinische Fakultät
Mathematisch-Naturwissenschaftlich-Technische Fakultät / Institut für Physik / Lehrstuhl für Experimentalphysik I
Medizinische Fakultät / Lehrstuhl für Physiologie
Fakultätsübergreifende Institute und Einrichtungen / Kompetenzzentrum für Innovative Technologien - Augsburg Centre for Innovative Technologies (ACIT)
Dewey Decimal Classification:6 Technik, Medizin, angewandte Wissenschaften / 61 Medizin und Gesundheit / 610 Medizin und Gesundheit
Licence (German):CC-BY 4.0: Creative Commons: Namensnennung (mit Print on Demand)