Open Access

Clonally expanded mitochondrial DNA (mtDNA) deletions accumulate with age in human substantia nigra (SN) and high levels cause respiratory chain deficiency. In other human tissues, mtDNA point mutations clonally expand with age. Here, the abundance of mtDNA point mutations within single SN neurons from aged controls was investigated. From 31 single cytochrome c oxidase normal SN neurons, only one clonally expanded mtDNA point mutation was identified, suggesting in these neurons mtDNA point mutations occur rarely, whereas mtDNA deletions are frequently observed. This contrasts observations in mitotic tissues and suggests that different forms of mtDNA maintenance may exist in these two cell types.

Recent experiments at ASDEX Upgrade have achieved advanced scenarios with high βN (>3) and confinement enhancement over ITER98(y, 2) scaling, HH98y2 = 1.1–1.5, in steady state. These discharges have been obtained in a modified divertor configuration for ASDEX Upgrade, allowing operation at higher triangularity, and with a changed neutral beam injection (NBI) system, for a more tangential, off-axis beam deposition. The figure of merit, βNHITER89-P, reaches up to 7.5 for several seconds in plasmas approaching stationary conditions. These advanced tokamak discharges have low magnetic shear in the centre, with q on-axis near 1, and edge safety factor, q95 in the range 3.3–4.5. This q-profile is sustained by the bootstrap current, NBI-driven current and fishbone activity in the core. The off-axis heating leads to a strong peaking of the density profile and impurity accumulation in the core. This can be avoided by adding some central heating from ion cyclotron resonance heating or electron cyclotron resonance heating, since the temperature profiles are stiff in this advanced scenario (no internal transport barrier). Using a combination of NBI and gas fuelling line, average densities up to 80–90% of the Greenwald density are achieved, maintaining good confinement. The best integrated results in terms of confinement, stability and ability to operate at high density are obtained in highly shaped configurations, near double null, with δ = 0.43. At the highest densities, a strong reduction of the edge localized mode activity similar to type II activity is observed, providing a steady power load on the divertor, in the range of 6 MW m−2, despite the high input power used (>10 MW).

Planted forests are critical to climate change mitigation and constitute a major supplier of timber/non-timber products and other ecosystem services. Globally, approximately 36% of planted forest area is located in East Asia. However, reliable records of the geographic distribution and tree species composition of these planted forests remain very limited. Here, based on extensive in situ and remote sensing data, as well as an ensemble modeling approach, we present the first spatial database of planted forests for East Asia, which consists of maps of the geographic distribution of planted forests and associated dominant tree genera. Of the predicted planted forest areas in East Asia (948,863 km2), China contributed 87%, most of which is located in the lowland tropical/subtropical regions, and Sichuan Basin. With 95% accuracy and an F1 score of 0.77, our spatially-continuous maps of planted forests enable accurate quantification of the role of planted forests in climate change mitigation. Our findings inform effective decision-making in forest conservation, management, and global restoration projects.